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Image Search Results
Journal: bioRxiv
Article Title: Balancing activation and costimulation of CAR tunes signaling dynamics and enhances therapeutic potency
doi: 10.1101/2022.03.01.482445
Figure Lengend Snippet: CD19-1XX CAR-T cells show better anti-tumor activity in a pancreatic tumor model in vivo. (a) Timeline of of CD19 CAR-T treatment in mouse model. (b) Panc-1 CD19+ -bearing mice were treated with 1 × 10 6 CD19 CAR-T cells as indicated and tumor burden (tumor volume) of mice was measured at indicated days (n=4 mice each group). For mouse experiments, Control, PBS. (c) Tumor tissue from mice treated with indicated CAR-T cells. (d) Tumor tissue from mice treated with indicated CAR-T cells was sliced and stained with antibodies against human CD4 and CD8 (n=4). The percentage of CD4 + (e) and CD8 + (f) T cells in tumor tissue from different groups of mice (n=4). All data are mean ± SEM.
Article Snippet: Anti-Human CD4 antibody (BD, Horizon, BUV395),
Techniques: Activity Assay, In Vivo, Staining
Journal: bioRxiv
Article Title: Balancing activation and costimulation of CAR tunes signaling dynamics and enhances therapeutic potency
doi: 10.1101/2022.03.01.482445
Figure Lengend Snippet: 1XX modification of AXL CAR enhance its anti-tumor activity in a melanoma tumor model. (a) Timeline of of AXL CAR-T treatment in mouse model. (b) A375-bearing mice were treated with 2.5 × 10 6 AXL CAR-T cells and tumor burden (tumor volume) of mice were measured at indicated days (n=4, 5, 5 mice in respective groups). Control, PBS. (c) Tumor tissue of mice treated with indicated CAR-T cells (n=4, 5, 5). (d) The weight of tumors from different groups of mice (n=4, 5, 5). Number of CAR-T(e), CD4 + CAR-T cells (f) and CD8 + CAR-T cells (g) in spleen of mice 20 days post CAR-T infusion (n=5, 3). (h-k) Phenotype of CAR-T cells in the spleen of mice 20 days after CAR-T infusion as demonstrated by the percentage of T CM (CD45RA - CD62L + ) and T EFF (CD45RA + CD62L - ) cells (n=5, 3). All data are mean ± SEM.
Article Snippet: Anti-Human CD4 antibody (BD, Horizon, BUV395),
Techniques: Modification, Activity Assay